Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3606561..3607360 Replicon chromosome
Accession NZ_CP070098
Organism Escherichia coli strain FDAARGOS_1370

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag I6L00_RS17935 Protein ID WP_000347272.1
Coordinates 3606896..3607360 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag I6L00_RS17930 Protein ID WP_001307405.1
Coordinates 3606561..3606896 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L00_RS17915 (3602346) 3602346..3603116 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6L00_RS17920 (3603132) 3603132..3604466 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6L00_RS17925 (3604841) 3604841..3606412 + 1572 WP_001273738.1 galactarate dehydratase -
I6L00_RS17930 (3606561) 3606561..3606896 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6L00_RS17935 (3606896) 3606896..3607360 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6L00_RS17940 (3607415) 3607415..3608224 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6L00_RS17945 (3608473) 3608473..3609753 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6L00_RS17950 (3609776) 3609776..3610249 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6L00_RS17955 (3610260) 3610260..3611039 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6L00_RS17960 (3611029) 3611029..3611907 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
I6L00_RS17965 (3611925) 3611925..3612359 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3597036..3607360 10324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T193095 WP_000347272.1 NZ_CP070098:3606896-3607360 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T193095 NZ_CP092702:c1269275-1269168 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT193095 WP_001307405.1 NZ_CP070098:3606561-3606896 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT193095 NZ_CP092702:1269323-1269389 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References