Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3009210..3009937 Replicon chromosome
Accession NZ_CP070098
Organism Escherichia coli strain FDAARGOS_1370

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I6L00_RS15110 Protein ID WP_033550565.1
Coordinates 3009626..3009937 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6L00_RS15105 Protein ID WP_000126294.1
Coordinates 3009210..3009629 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L00_RS15085 (3004295) 3004295..3004822 - 528 WP_001078777.1 electron transport protein HydN -
I6L00_RS15090 (3004971) 3004971..3005981 - 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
I6L00_RS15095 (3006241) 3006241..3007698 + 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
I6L00_RS15100 (3007707) 3007707..3009131 + 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
I6L00_RS15105 (3009210) 3009210..3009629 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
I6L00_RS15110 (3009626) 3009626..3009937 - 312 WP_033550565.1 type II toxin-antitoxin system HigB family toxin Toxin
I6L00_RS15115 (3010104) 3010104..3010574 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
I6L00_RS15120 (3010567) 3010567..3010977 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I6L00_RS15125 (3010974) 3010974..3011741 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6L00_RS15130 (3011741) 3011741..3012283 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
I6L00_RS15135 (3012293) 3012293..3014002 - 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.19 Da        Isoelectric Point: 9.2034

>T193091 WP_033550565.1 NZ_CP070098:c3009937-3009626 [Escherichia coli]
MHIISQAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T193091 NZ_CP092702:c246239-245961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT193091 WP_000126294.1 NZ_CP070098:c3009629-3009210 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT193091 NZ_CP092702:c246502-246242 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References