Detailed information of TA system TA193069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 171281..171975
• Replicon: chromosome
• Accession: NZ_CP070098
• Organism: Escherichia coli strain FDAARGOS_1370

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: I6L00_RS01445
• Protein ID: WP_001521903.1
• Coordinates: 171577..171975 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6L00_RS01440
• Protein ID: WP_000554758.1
• Coordinates: 171281..171574 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L00_RS01415 (166396)	166396..167145	+	750	WP_016233951.1	C40 family peptidase	-
I6L00_RS01420 (167202)	167202..167555	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
I6L00_RS01425 (167634)	167634..169346	-	1713	Protein_145	flagellar biosynthesis protein FlhA	-
I6L00_RS01430 (169318)	169318..170103	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
I6L00_RS01435 (170174)	170174..171229	+	1056	WP_001226177.1	DNA polymerase IV	-
I6L00_RS01440 (171281)	171281..171574	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6L00_RS01445 (171577)	171577..171975	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6L00_RS01450 (171985)	171985..172437	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
I6L00_RS01455 (172682)	172682..172888	+	207	Protein_151	RtcB family protein	-
I6L00_RS01460 (172884)	172884..173236	+	353	Protein_152	peptide chain release factor H	-
I6L00_RS01465 (173293)	173293..174750	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
I6L00_RS01470 (175011)	175011..175469	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (176065)	176065..176145	+	81	NuclAT_11	-	-
- (176065)	176065..176145	+	81	NuclAT_11	-	-
- (176065)	176065..176145	+	81	NuclAT_11	-	-
- (176065)	176065..176145	+	81	NuclAT_11	-	-
I6L00_RS01475 (175561)	175561..176805	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T193069 WP_001521903.1 NZ_CP070098:171577-171975 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193069 NZ_CP092680:c7443-7195 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193069 WP_000554758.1 NZ_CP070098:171281-171574 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193069 NZ_CP092680:c7201-6878 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGGGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCAT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTTGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3