Detailed information of TA system TA193004

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34393..34662
• Replicon: plasmid unnamed1
• Accession: NZ_CP070078
• Organism: Escherichia coli strain FDAARGOS_1371

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6L01_RS24170
• Protein ID: WP_001372321.1
• Coordinates: 34537..34662 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34393..34458 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L01_RS24800 (29435)	29435..29860	-	426	Protein_33	hypothetical protein	-
I6L01_RS24140 (29930)	29930..30136	+	207	WP_199381625.1	single-stranded DNA-binding protein	-
I6L01_RS24145 (30162)	30162..30701	+	540	WP_204628262.1	single-stranded DNA-binding protein	-
I6L01_RS24150 (30763)	30763..30996	+	234	WP_000006030.1	DUF905 family protein	-
I6L01_RS24155 (31061)	31061..33019	+	1959	WP_199381624.1	ParB/RepB/Spo0J family partition protein	-
I6L01_RS24160 (33074)	33074..33508	+	435	WP_000845908.1	conjugation system SOS inhibitor PsiB	-
I6L01_RS24165 (33505)	33505..34267	+	763	Protein_39	plasmid SOS inhibition protein A	-
- (34393)	34393..34458	+	66	NuclAT_1	-	-
- (34393)	34393..34458	-	66	NuclAT_0	-	Antitoxin
- (34393)	34393..34458	-	66	NuclAT_0	-	Antitoxin
- (34236)	34236..34460	+	225	NuclAT_0	-	-
- (34236)	34236..34460	+	225	NuclAT_0	-	-
- (34236)	34236..34460	+	225	NuclAT_0	-	-
- (34236)	34236..34460	+	225	NuclAT_0	-	-
- (34236)	34236..34460	-	225	NuclAT_0	-	-
I6L01_RS24805 (34446)	34446..34595	+	150	Protein_40	plasmid maintenance protein Mok	-
I6L01_RS24170 (34537)	34537..34662	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6L01_RS24810 (34963)	34963..35260	-	298	Protein_42	hypothetical protein	-
I6L01_RS24175 (35330)	35330..35602	+	273	WP_225556386.1	single-stranded DNA-binding protein	-
I6L01_RS24180 (35556)	35556..35879	+	324	WP_000533253.1	hypothetical protein	-
I6L01_RS24185 (35938)	35938..36180	+	243	WP_000540591.1	hypothetical protein	-
I6L01_RS24815 (36380)	36380..36803	-	424	Protein_46	hypothetical protein	-
I6L01_RS24190 (36873)	36873..37079	+	207	WP_000547974.1	hypothetical protein	-
I6L01_RS24195 (37103)	37103..37399	+	297	WP_021553188.1	hypothetical protein	-
I6L01_RS24200 (37510)	37510..38331	+	822	WP_204628259.1	DUF932 domain-containing protein	-
I6L01_RS24205 (38627)	38627..39136	-	510	WP_021553190.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T193004 WP_001372321.1 NZ_CP070078:34537-34662 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T193004 NZ_CP092647:c60919-59597 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
ATTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT193004 NZ_CP070078:c34458-34393 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin