Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3309426..3310153 Replicon chromosome
Accession NZ_CP070077
Organism Escherichia coli strain FDAARGOS_1371

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I6L01_RS15555 Protein ID WP_033550565.1
Coordinates 3309426..3309737 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6L01_RS15560 Protein ID WP_000126294.1
Coordinates 3309734..3310153 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L01_RS15530 (3305361) 3305361..3307070 + 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -
I6L01_RS15535 (3307080) 3307080..3307622 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
I6L01_RS15540 (3307622) 3307622..3308389 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6L01_RS15545 (3308386) 3308386..3308796 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I6L01_RS15550 (3308789) 3308789..3309259 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
I6L01_RS15555 (3309426) 3309426..3309737 + 312 WP_033550565.1 type II toxin-antitoxin system HigB family toxin Toxin
I6L01_RS15560 (3309734) 3309734..3310153 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
I6L01_RS15565 (3310232) 3310232..3311656 - 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
I6L01_RS15570 (3311665) 3311665..3313122 - 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
I6L01_RS15575 (3313382) 3313382..3314392 + 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
I6L01_RS15580 (3314541) 3314541..3315068 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.19 Da        Isoelectric Point: 9.2034

>T192992 WP_033550565.1 NZ_CP070077:3309426-3309737 [Escherichia coli]
MHIISQAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T192992 NZ_CP092639:2591677-2591784 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT192992 WP_000126294.1 NZ_CP070077:3309734-3310153 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT192992 NZ_CP092639:c2591630-2591564 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References