Detailed information of TA system TA192987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1935554..1936156
• Replicon: chromosome
• Accession: NZ_CP070077
• Organism: Escherichia coli strain FDAARGOS_1371

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6L01_RS09105
• Protein ID: WP_000897305.1
• Coordinates: 1935845..1936156 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L01_RS09100
• Protein ID: WP_000356395.1
• Coordinates: 1935554..1935844 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L01_RS09070 (1931177)	1931177..1932079	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6L01_RS09075 (1932076)	1932076..1932711	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6L01_RS09080 (1932708)	1932708..1933637	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I6L01_RS09085 (1933819)	1933819..1934061	-	243	WP_021523315.1	hypothetical protein	-
I6L01_RS09090 (1934280)	1934280..1934498	-	219	WP_001295676.1	CopG family transcriptional regulator	-
I6L01_RS09095 (1934917)	1934917..1935195	-	279	WP_001306650.1	hypothetical protein	-
I6L01_RS09100 (1935554)	1935554..1935844	-	291	WP_000356395.1	NadS family protein	Antitoxin
I6L01_RS09105 (1935845)	1935845..1936156	-	312	WP_000897305.1	hypothetical protein	Toxin
I6L01_RS09110 (1936385)	1936385..1937293	+	909	WP_001306651.1	alpha/beta hydrolase	-
I6L01_RS24655 (1937462)	1937462..1938376	-	915	WP_077634137.1	transposase	-
I6L01_RS24660 (1938389)	1938389..1939276	-	888	Protein_1785	hypothetical protein	-
I6L01_RS09120 (1939765)	1939765..1940223	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1939765..1940223	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T192987 WP_000897305.1 NZ_CP070077:c1936156-1935845 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192987 NZ_CP092639:1775316-1775570 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT192987 WP_000356395.1 NZ_CP070077:c1935844-1935554 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192987 NZ_CP092639:1775068-1775319 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7