Detailed information of TA system TA192974

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44820..45074
• Replicon: plasmid unnamed3
• Accession: NZ_CP070073
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I6K17_RS26625
• Protein ID: WP_001312851.1
• Coordinates: 44925..45074 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	44820..44876 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS26590 (40757)	40757..41503	+	747	WP_000205764.1	conjugal transfer pilus acetylase TraX	-
I6K17_RS26595 (41557)	41557..42117	+	561	WP_000139341.1	fertility inhibition protein FinO	-
I6K17_RS26600 (42248)	42248..42460	+	213	WP_032161968.1	hypothetical protein	-
I6K17_RS26605 (42705)	42705..43166	+	462	WP_001233850.1	thermonuclease family protein	-
I6K17_RS26610 (43212)	43212..43421	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
I6K17_RS26615 (43459)	43459..44049	+	591	WP_000766807.1	DUF2726 domain-containing protein	-
I6K17_RS26620 (44204)	44204..44653	+	450	WP_053287837.1	hypothetical protein	-
- (44820)	44820..44876	-	57	NuclAT_1	-	Antitoxin
- (44820)	44820..44876	-	57	NuclAT_1	-	Antitoxin
- (44820)	44820..44876	-	57	NuclAT_1	-	Antitoxin
- (44820)	44820..44876	-	57	NuclAT_1	-	Antitoxin
I6K17_RS26625 (44925)	44925..45074	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
I6K17_RS26630 (45358)	45358..45618	+	261	WP_000083819.1	replication regulatory protein RepA	-
I6K17_RS27985 (45644)	45644..45877	-	234	WP_225103364.1	replication protein RepA	-
I6K17_RS27990 (45868)	45868..45915	+	48	WP_077822033.1	hypothetical protein	-
I6K17_RS26640 (45908)	45908..46765	+	858	WP_021534913.1	incFII family plasmid replication initiator RepA	-
I6K17_RS27995 (47676)	47676..47813	+	138	Protein_62	XRE family transcriptional regulator	-
I6K17_RS26650 (47833)	47833..48096	+	264	WP_001089474.1	hypothetical protein	-
I6K17_RS26655 (48086)	48086..48385	+	300	WP_000119542.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6K17_RS26660 (48618)	48618..49085	-	468	WP_247126557.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68898	68898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T192974 WP_001312851.1 NZ_CP070073:44925-45074 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T192974 NZ_CP092635:3997519-3997881 [Enterobacter cloacae]
ATGTGGAACGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAAAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT192974 NZ_CP070073:c44876-44820 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin