Detailed information of TA system TA192971

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2694..2963
• Replicon: plasmid unnamed3
• Accession: NZ_CP070073
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K17_RS26375
• Protein ID: WP_001372321.1
• Coordinates: 2838..2963 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	2694..2759 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS26360	1375..1809	+	435	WP_021534892.1	conjugation system SOS inhibitor PsiB	-
I6K17_RS26365	1806..2568	+	763	Protein_2	plasmid SOS inhibition protein A	-
-	2537..2761	+	225	NuclAT_0	-	-
-	2537..2761	+	225	NuclAT_0	-	-
-	2537..2761	+	225	NuclAT_0	-	-
-	2537..2761	+	225	NuclAT_0	-	-
I6K17_RS26370	2546..2725	-	180	WP_001309233.1	hypothetical protein	-
-	2694..2759	-	66	-	-	Antitoxin
I6K17_RS27970	2747..2896	+	150	Protein_4	plasmid maintenance protein Mok	-
I6K17_RS26375	2838..2963	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K17_RS27975	3415..3588	-	174	Protein_6	hypothetical protein	-
I6K17_RS26380	3889..4176	+	288	WP_000107526.1	hypothetical protein	-
I6K17_RS26385	4295..5116	+	822	WP_032172795.1	DUF932 domain-containing protein	-
I6K17_RS26390	5412..6014	-	603	WP_000243703.1	transglycosylase SLT domain-containing protein	-
I6K17_RS26395	6345..6728	+	384	WP_001151565.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K17_RS26400	6922..7614	+	693	WP_021534889.1	PAS domain-containing protein	-
I6K17_RS26405	7719..7946	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68898	68898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192971 WP_001372321.1 NZ_CP070073:2838-2963 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192971 NZ_CP092635:c1207910-1207611 [Enterobacter cloacae]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGGAGATCGAGCAATGTTATTCCTTCAAATCT
GGAAACCGTTCTTGCAAGAAAGCTCGACCTCATCAATGCAGCCATCTCGCATCGGGATTTGCGTTCACCACCGGGCAATA
TGTTTGAAGCATTGAATCCGCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
GCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT192971 NZ_CP070073:c2759-2694 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin