Detailed information of TA system TA192968

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108451..108876
• Replicon: plasmid unnamed2
• Accession: NZ_CP070072
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K17_RS26175
• Protein ID: WP_223195199.1
• Coordinates: 108754..108876 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	108451..108662 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS26140 (103655)	103655..104080	-	426	WP_071791129.1	hypothetical protein	-
I6K17_RS26145 (104150)	104150..104356	+	207	WP_000547940.1	hypothetical protein	-
I6K17_RS26150 (104382)	104382..104921	+	540	WP_076838608.1	single-stranded DNA-binding protein	-
I6K17_RS26155 (104979)	104979..105212	+	234	WP_001553739.1	DUF905 family protein	-
I6K17_RS26160 (105276)	105276..107234	+	1959	WP_204609223.1	ParB/RepB/Spo0J family partition protein	-
I6K17_RS26165 (107289)	107289..107723	+	435	WP_076838612.1	conjugation system SOS inhibitor PsiB	-
I6K17_RS26170 (107720)	107720..108506	+	787	Protein_111	plasmid SOS inhibition protein A	-
- (108451)	108451..108662	+	212	NuclAT_0	-	Antitoxin
- (108451)	108451..108662	+	212	NuclAT_0	-	Antitoxin
- (108451)	108451..108662	+	212	NuclAT_0	-	Antitoxin
- (108451)	108451..108662	+	212	NuclAT_0	-	Antitoxin
I6K17_RS27960 (108696)	108696..108809	+	114	Protein_112	DUF5431 family protein	-
I6K17_RS26175 (108754)	108754..108876	+	123	WP_223195199.1	Hok/Gef family protein	Toxin
I6K17_RS26180 (109177)	109177..109474	-	298	Protein_114	hypothetical protein	-
I6K17_RS26185 (109775)	109775..110062	+	288	Protein_115	hypothetical protein	-
I6K17_RS26190 (110181)	110181..111002	+	822	WP_001234468.1	DUF932 domain-containing protein	-
I6K17_RS26195 (111298)	111298..111900	-	603	Protein_117	transglycosylase SLT domain-containing protein	-
I6K17_RS26200 (112222)	112222..112605	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K17_RS26205 (112795)	112795..113481	+	687	WP_000332490.1	PAS domain-containing protein	-
I6K17_RS26210 (113575)	113575..113802	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..136908	136908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T192968 WP_223195199.1 NZ_CP070072:108754-108876 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T192968 NZ_CP092635:133834-135159 [Enterobacter cloacae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Nucleotide

• Length: 212 bp

>AT192968 NZ_CP070072:108451-108662 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin