Detailed information of TA system TA192953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3617491..3618093
• Replicon: chromosome
• Accession: NZ_CP070071
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6K17_RS18410
• Protein ID: WP_000897305.1
• Coordinates: 3617782..3618093 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K17_RS18405
• Protein ID: WP_000356397.1
• Coordinates: 3617491..3617781 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS18385 (3613993)	3613993..3614895	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K17_RS18390 (3614892)	3614892..3615527	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K17_RS18395 (3615524)	3615524..3616453	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K17_RS18400 (3616668)	3616668..3616886	-	219	WP_001295676.1	CopG family transcriptional regulator	-
I6K17_RS18405 (3617491)	3617491..3617781	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K17_RS18410 (3617782)	3617782..3618093	-	312	WP_000897305.1	hypothetical protein	Toxin
I6K17_RS18415 (3618322)	3618322..3619230	+	909	WP_001318161.1	alpha/beta hydrolase	-
I6K17_RS18420 (3619294)	3619294..3620235	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K17_RS18425 (3620280)	3620280..3620717	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K17_RS18430 (3620714)	3620714..3621586	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K17_RS18435 (3621580)	3621580..3622179	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T192953 WP_000897305.1 NZ_CP070071:c3618093-3617782 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192953 NZ_CP092632:1780549-1780881 [Klebsiella variicola subsp. variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192953 WP_000356397.1 NZ_CP070071:c3617781-3617491 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192953 NZ_CP092632:1780291-1780548 [Klebsiella variicola subsp. variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7