Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2432028..2432707 Replicon chromosome
Accession NZ_CP070071
Organism Escherichia coli strain FDAARGOS_1287

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag I6K17_RS12810 Protein ID WP_000057523.1
Coordinates 2432405..2432707 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K17_RS12805 Protein ID WP_000806442.1
Coordinates 2432028..2432369 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K17_RS12795 (2428271) 2428271..2429203 - 933 WP_000883024.1 glutaminase A -
I6K17_RS12800 (2429466) 2429466..2431970 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
I6K17_RS12805 (2432028) 2432028..2432369 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K17_RS12810 (2432405) 2432405..2432707 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K17_RS12815 (2432840) 2432840..2433634 + 795 WP_000365161.1 TraB/GumN family protein -
I6K17_RS12820 (2433838) 2433838..2434317 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K17_RS12825 (2434354) 2434354..2435910 - 1557 WP_204609064.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase UshA -
I6K17_RS12830 (2436128) 2436128..2437348 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T192948 WP_000057523.1 NZ_CP070071:c2432707-2432405 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T192948 NZ_CP092631:c2238286-2238110 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTTCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT192948 WP_000806442.1 NZ_CP070071:c2432369-2432028 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT192948 NZ_CP092631:2238069-2238169 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References