Detailed information of TA system TA192925

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31617..31881
• Replicon: plasmid unnamed1
• Accession: NZ_CP070070
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: I6K17_RS00170
• Protein ID: WP_001387489.1
• Coordinates: 31617..31769 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31819..31881 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS00145 (26894)	26894..29062	+	2169	WP_000698357.1	DotA/TraY family protein	-
I6K17_RS00150 (29136)	29136..29786	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
I6K17_RS00155 (29858)	29858..30067	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
I6K17_RS00160 (30459)	30459..30635	+	177	WP_001054898.1	hypothetical protein	-
I6K17_RS28035 (30700)	30700..30795	-	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
I6K17_RS00165 (31294)	31294..31545	+	252	WP_001291964.1	hypothetical protein	-
I6K17_RS00170 (31617)	31617..31769	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (31819)	31819..31881	+	63	NuclAT_0	-	Antitoxin
- (31819)	31819..31881	+	63	NuclAT_0	-	Antitoxin
- (31819)	31819..31881	+	63	NuclAT_0	-	Antitoxin
- (31819)	31819..31881	+	63	NuclAT_0	-	Antitoxin
I6K17_RS00175 (32061)	32061..33269	+	1209	WP_039005623.1	IncI1-type conjugal transfer protein TrbA	-
I6K17_RS00180 (33288)	33288..34358	+	1071	WP_039005625.1	IncI1-type conjugal transfer protein TrbB	-
I6K17_RS00185 (34351)	34351..36642	+	2292	WP_072742854.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..112030	112030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T192925 WP_001387489.1 NZ_CP070070:c31769-31617 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T192925 NZ_CP092627:c1002716-1002189 [Salmonella enterica subsp. enterica serovar Thompson]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT192925 NZ_CP070070:31819-31881 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin