Detailed information of TA system TA192921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4793155..4793757
• Replicon: chromosome
• Accession: NZ_CP070066
• Organism: Escherichia coli strain FDAARGOS_1296

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K26_RS23100
• Protein ID: WP_000897302.1
• Coordinates: 4793446..4793757 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K26_RS23095
• Protein ID: WP_000356397.1
• Coordinates: 4793155..4793445 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K26_RS23070 (4789228)	4789228..4790130	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K26_RS23075 (4790127)	4790127..4790762	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K26_RS23080 (4790759)	4790759..4791688	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K26_RS23085 (4791904)	4791904..4792122	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K26_RS23090 (4792518)	4792518..4792796	-	279	WP_001296612.1	hypothetical protein	-
I6K26_RS23095 (4793155)	4793155..4793445	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K26_RS23100 (4793446)	4793446..4793757	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K26_RS23105 (4793986)	4793986..4794894	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6K26_RS23110 (4794958)	4794958..4795899	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K26_RS23115 (4795944)	4795944..4796381	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K26_RS23120 (4796378)	4796378..4797250	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K26_RS23125 (4797244)	4797244..4797843	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192921 WP_000897302.1 NZ_CP070066:c4793757-4793446 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192921 NZ_CP092627:205201-205686 [Salmonella enterica subsp. enterica serovar Thompson]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192921 WP_000356397.1 NZ_CP070066:c4793445-4793155 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192921 NZ_CP092627:204926-205213 [Salmonella enterica subsp. enterica serovar Thompson]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: S1P416