Detailed information of TA system TA192916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3886282..3886976
• Replicon: chromosome
• Accession: NZ_CP070066
• Organism: Escherichia coli strain FDAARGOS_1296

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K26_RS18895
• Protein ID: WP_001263500.1
• Coordinates: 3886282..3886680 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K26_RS18900
• Protein ID: WP_000554758.1
• Coordinates: 3886683..3886976 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K26_RS18870 (3881647)	3881647..3882105	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
I6K26_RS18875 (3882366)	3882366..3883823	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K26_RS18880 (3883880)	3883880..3884494	-	615	WP_000602123.1	peptide chain release factor H	-
I6K26_RS18885 (3884491)	3884491..3885630	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K26_RS18890 (3885820)	3885820..3886272	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K26_RS18895 (3886282)	3886282..3886680	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K26_RS18900 (3886683)	3886683..3886976	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K26_RS18905 (3887028)	3887028..3888083	-	1056	WP_001226168.1	DNA polymerase IV	-
I6K26_RS18910 (3888154)	3888154..3888939	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K26_RS18915 (3888911)	3888911..3890623	+	1713	Protein_3736	flagellar biosynthesis protein FlhA	-
I6K26_RS18920 (3890943)	3890943..3891440	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3886282..3906636	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T192916 WP_001263500.1 NZ_CP070066:c3886680-3886282 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192916 NZ_CP092589:c4734070-4733786 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192916 WP_000554758.1 NZ_CP070066:c3886976-3886683 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192916 NZ_CP092589:c4734302-4734060 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3