Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1994155..1994657 Replicon chromosome
Accession NZ_CP070066
Organism Escherichia coli strain FDAARGOS_1296

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K26_RS09575 Protein ID WP_000767822.1
Coordinates 1994403..1994657 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K26_RS09570 Protein ID WP_001259255.1
Coordinates 1994155..1994406 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K26_RS09545 (1989333) 1989333..1990400 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6K26_RS09550 (1990400) 1990400..1991470 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6K26_RS09555 (1991467) 1991467..1992771 - 1305 WP_001297912.1 histidinol dehydrogenase -
I6K26_RS09560 (1992777) 1992777..1993676 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K26_RS09565 (1993822) 1993822..1993872 - 51 WP_001364200.1 his operon leader peptide -
I6K26_RS09570 (1994155) 1994155..1994406 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K26_RS09575 (1994403) 1994403..1994657 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K26_RS09580 (1994740) 1994740..1995564 + 825 WP_000754733.1 SDR family oxidoreductase -
I6K26_RS09585 (1995610) 1995610..1996539 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K26_RS09590 (1996754) 1996754..1996816 + 63 WP_010723108.1 membrane protein YoeI -
I6K26_RS09595 (1996806) 1996806..1998164 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T192902 WP_000767822.1 NZ_CP070066:1994403-1994657 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T192902 NZ_CP092588:c281291-281013 [Vibrio gazogenes]
ATGATTTTATGGGAGGAAGAATCTCTCAATGATCGCGAAAAGATCTTCGAGTTTCTTTACGATTTTAACCCAGACGCTGC
CGAGAAAACCGATGAACTTATCGAGGCCAAAGTCGAAAACCTTTTGGAACAACCTCTTATGGGGGTACAGCGTGATGGCA
TTCGGGGACGACTTCTGAGCATTTCTGAGATATCAATGATCGTCTCGTACTGGGTTGAAGGTGATATCGTCCATGTTATG
CGCGTACTACACCAAAAACAAAAATTCCCTGCTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT192902 WP_001259255.1 NZ_CP070066:1994155-1994406 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT192902 NZ_CP092588:c281572-281288 [Vibrio gazogenes]
ATGGACACTAGAATTCAATTTCGTGTTGATGAGGAAACAAAGCGTTTAGCCCAACAAATGGCTGAAAGCCAAGGTCGAAC
CTTAAGCGACGCTTGCCGTGAACTTACCGAACAACTCGCTGAGCAACAACGAAAAACGTTATCTCATGATGCATGGCTTA
CTGACCAAGTCAACCTAGCTTTTGAGAAGTTTGACTCAGGAAAAGCTGTTTTCACTGACCATAACGCTGCGAAATCTCGA
ATGACTGAACGTAAGGCTAAGATTCGCAACCGAGGCAAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References