Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2739786..2740348 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6L02_RS13035 Protein ID WP_000605675.1
Coordinates 2740070..2740348 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6L02_RS13030 Protein ID WP_000781370.1
Coordinates 2739786..2740070 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS13015 (2734967) 2734967..2738014 + 3048 WP_077627348.1 formate dehydrogenase-N subunit alpha -
I6L02_RS13020 (2738027) 2738027..2738911 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6L02_RS13025 (2738904) 2738904..2739557 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6L02_RS13030 (2739786) 2739786..2740070 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6L02_RS13035 (2740070) 2740070..2740348 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L02_RS13040 (2740534) 2740534..2741544 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6L02_RS13045 (2741678) 2741678..2743375 - 1698 WP_000433462.1 malate dehydrogenase -
I6L02_RS13050 (2743531) 2743531..2743668 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6L02_RS13055 (2743770) 2743770..2743985 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6L02_RS13060 (2744330) 2744330..2744761 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192882 WP_000605675.1 NZ_CP070063:c2740348-2740070 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192882 NZ_CP092574:2994621-2994764 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192882 WP_000781370.1 NZ_CP070063:c2740070-2739786 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192882 NZ_CP092574:c2994952-2994697 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATAAT
AGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACTTTTTGGATTATTTAA
AAATAACCGTACTGGTCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGTGCAATCAAAGCA
ATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References