Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1728775..1729454 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6L02_RS07955 Protein ID WP_000057524.1
Coordinates 1728775..1729077 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6L02_RS07960 Protein ID WP_000806442.1
Coordinates 1729113..1729454 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS07930 (1724151) 1724151..1725803 + 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
I6L02_RS07935 (1725841) 1725841..1726344 - 504 WP_000667000.1 hypothetical protein -
I6L02_RS07940 (1726341) 1726341..1727141 - 801 WP_000439798.1 hypothetical protein -
I6L02_RS07945 (1727165) 1727165..1727644 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6L02_RS07950 (1727848) 1727848..1728642 - 795 WP_000365177.1 TraB/GumN family protein -
I6L02_RS07955 (1728775) 1728775..1729077 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L02_RS07960 (1729113) 1729113..1729454 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6L02_RS07965 (1729512) 1729512..1732016 - 2505 WP_204673708.1 copper-exporting P-type ATPase CopA -
I6L02_RS07970 (1732279) 1732279..1733211 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T192877 WP_000057524.1 NZ_CP070063:1728775-1729077 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T192877 NZ_CP092574:2640307-2640651 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ATGTAAAAGACCATGTGGATATAATGGCATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAAGCTGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT192877 WP_000806442.1 NZ_CP070063:1729113-1729454 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT192877 NZ_CP092574:2639953-2640288 [Enterococcus faecalis]
ATGACAGTGTTTGATAGGATCAAATTATTAGCAAAAGAGAGAGGAAAATCCTTAAATCGAATCGAAGAAGAATTAGGTTT
ACCAAAAAATGTTCTGTATCGTATGAAAACTTCCGATAATCCTACTAAAGATAGACTAGAAACTTTAGCTAATTACTTCA
ATGTATCGGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAATTCTGATGATTTAGATGAT
GTACTGGATAACGTCATGAGTTTTGACGGTGAACCACTTGATGATCATGACAGAGAAGTTATCCGTGCATATTTAAAGGG
TAGATTCGGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References