Detailed information of TA system TA192875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1500911..1501605
• Replicon: chromosome
• Accession: NZ_CP070063
• Organism: Escherichia coli strain FDAARGOS_1372

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6L02_RS06910
• Protein ID: WP_001263500.1
• Coordinates: 1501207..1501605 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6L02_RS06905
• Protein ID: WP_000554758.1
• Coordinates: 1500911..1501204 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L02_RS06885 (1496647)	1496647..1497144	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6L02_RS06890 (1497264)	1497264..1498976	-	1713	Protein_1348	flagellar biosynthesis protein FlhA	-
I6L02_RS06895 (1498948)	1498948..1499733	+	786	WP_000207543.1	putative lateral flagellar export/assembly protein LafU	-
I6L02_RS06900 (1499804)	1499804..1500859	+	1056	WP_001226162.1	DNA polymerase IV	-
I6L02_RS06905 (1500911)	1500911..1501204	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6L02_RS06910 (1501207)	1501207..1501605	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6L02_RS06915 (1501615)	1501615..1502067	+	453	WP_001059897.1	GNAT family N-acetyltransferase	-
I6L02_RS06920 (1502257)	1502257..1503396	+	1140	WP_000521564.1	RNA ligase RtcB family protein	-
I6L02_RS06925 (1503393)	1503393..1504007	+	615	WP_000602123.1	peptide chain release factor H	-
I6L02_RS06930 (1504064)	1504064..1505521	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6L02_RS06935 (1505782)	1505782..1506240	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1475051..1502067	27016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T192875 WP_001263500.1 NZ_CP070063:1501207-1501605 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192875 NZ_CP092574:1642336-1642677 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192875 WP_000554758.1 NZ_CP070063:1500911-1501204 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192875 NZ_CP092574:1642106-1642336 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAACAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3