Detailed information of TA system TA192869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 629023..629625
• Replicon: chromosome
• Accession: NZ_CP070063
• Organism: Escherichia coli strain FDAARGOS_1372

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6L02_RS02905
• Protein ID: WP_000897302.1
• Coordinates: 629023..629334 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L02_RS02910
• Protein ID: WP_000356397.1
• Coordinates: 629335..629625 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L02_RS02875 (624053)	624053..624838	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
I6L02_RS02880 (624937)	624937..625536	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6L02_RS02885 (625530)	625530..626402	+	873	WP_039059842.1	virulence factor BrkB family protein	-
I6L02_RS02890 (626399)	626399..626836	+	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
I6L02_RS02895 (626881)	626881..627822	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6L02_RS02900 (627886)	627886..628794	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6L02_RS02905 (629023)	629023..629334	+	312	WP_000897302.1	hypothetical protein	Toxin
I6L02_RS02910 (629335)	629335..629625	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6L02_RS02915 (629984)	629984..630262	+	279	WP_001296612.1	hypothetical protein	-
I6L02_RS02920 (630658)	630658..630876	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6L02_RS02925 (631092)	631092..632021	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6L02_RS02930 (632018)	632018..632653	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6L02_RS02935 (632650)	632650..633552	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192869 WP_000897302.1 NZ_CP070063:629023-629334 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192869 NZ_CP092567:c2541003-2540737 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192869 WP_000356397.1 NZ_CP070063:629335-629625 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192869 NZ_CP092567:c2541260-2541003 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: S1P416