Detailed information of TA system TA192858

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22935..23204
• Replicon: plasmid unnamed1
• Accession: NZ_CP070049
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K20_RS24650
• Protein ID: WP_001372321.1
• Coordinates: 23079..23204 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22935..23000 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS25355 (17977)	17977..18195	+	219	WP_074017426.1	hypothetical protein	-
I6K20_RS24620 (18704)	18704..19243	+	540	WP_000290847.1	single-stranded DNA-binding protein	-
I6K20_RS24625 (19305)	19305..19538	+	234	WP_000006030.1	DUF905 family protein	-
I6K20_RS24630 (19603)	19603..21561	+	1959	WP_000117213.1	ParB/RepB/Spo0J family partition protein	-
I6K20_RS24635 (21616)	21616..22050	+	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
I6K20_RS24640 (22047)	22047..22809	+	763	Protein_30	plasmid SOS inhibition protein A	-
I6K20_RS24645 (22778)	22778..22966	-	189	WP_001336239.1	hypothetical protein	-
- (22935)	22935..23000	+	66	NuclAT_1	-	-
- (22935)	22935..23000	-	66	NuclAT_0	-	Antitoxin
- (22935)	22935..23000	-	66	NuclAT_0	-	Antitoxin
- (22778)	22778..23002	+	225	NuclAT_0	-	-
- (22778)	22778..23002	+	225	NuclAT_0	-	-
- (22778)	22778..23002	+	225	NuclAT_0	-	-
- (22778)	22778..23002	+	225	NuclAT_0	-	-
- (22778)	22778..23002	-	225	NuclAT_0	-	-
I6K20_RS25360 (22988)	22988..23137	+	150	Protein_32	plasmid maintenance protein Mok	-
I6K20_RS24650 (23079)	23079..23204	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K20_RS25365 (23658)	23658..23831	-	174	Protein_34	hypothetical protein	-
I6K20_RS24655 (24131)	24131..24418	+	288	WP_000107526.1	hypothetical protein	-
I6K20_RS24660 (24537)	24537..25358	+	822	WP_204693239.1	DUF932 domain-containing protein	-
I6K20_RS24665 (25653)	25653..26255	-	603	WP_023908348.1	transglycosylase SLT domain-containing protein	-
I6K20_RS24670 (26578)	26578..26961	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K20_RS24675 (27155)	27155..27826	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
I6K20_RS24680 (27963)	27963..28190	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192858 WP_001372321.1 NZ_CP070049:23079-23204 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192858 NZ_CP092565:1962790-1963251 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTTGAAGCGCACAATTACGGTGTACGTAATTTATATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT192858 NZ_CP070049:c23000-22935 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin