Detailed information of TA system TA192856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4822335..4822985
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6K20_RS23195
• Protein ID: WP_000263532.1
• Coordinates: 4822335..4822676 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: I6K20_RS23200
• Protein ID: WP_000212552.1
• Coordinates: 4822686..4822985 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS23170 (4817370)	4817370..4818470	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
I6K20_RS23175 (4818470)	4818470..4818769	+	300	WP_000867281.1	rod-binding protein	-
I6K20_RS23180 (4818945)	4818945..4820321	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
I6K20_RS23185 (4820336)	4820336..4821265	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
I6K20_RS23190 (4821282)	4821282..4822259	+	978	WP_001195938.1	hypothetical protein	-
I6K20_RS23195 (4822335)	4822335..4822676	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K20_RS23200 (4822686)	4822686..4822985	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K20_RS23205 (4823043)	4823043..4823885	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
I6K20_RS23210 (4824371)	4824371..4825285	+	915	WP_000949083.1	lateral flagellin LafA	-
I6K20_RS23215 (4825490)	4825490..4826806	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
I6K20_RS23220 (4826829)	4826829..4827221	+	393	WP_000285322.1	flagellar export chaperone FliS	-
I6K20_RS23225 (4827226)	4827226..4827537	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T192856 WP_000263532.1 NZ_CP070048:4822335-4822676 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T192856 NZ_CP092565:c852087-851899 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT192856 WP_000212552.1 NZ_CP070048:4822686-4822985 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT192856 NZ_CP092565:c852376-852116 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7