Detailed information of TA system TA192850

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4432474..4432886
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: I6K20_RS21395
• Protein ID: WP_000132601.1
• Coordinates: 4432474..4432815 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4432810..4432886 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS21385 (4429887)	4429887..4430933	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
I6K20_RS21390 (4430933)	4430933..4432312	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
I6K20_RS21395 (4432474)	4432474..4432815	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
- (4432810)	4432810..4432886	+	77	NuclAT_12	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_12	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_12	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_12	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_13	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_13	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_13	-	Antitoxin
- (4432810)	4432810..4432886	+	77	NuclAT_13	-	Antitoxin
I6K20_RS21400 (4433043)	4433043..4434437	-	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
I6K20_RS21405 (4434434)	4434434..4436023	-	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4420183..4434437	14254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T192850 WP_000132601.1 NZ_CP070048:c4432815-4432474 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T192850 NZ_CP092563:c1889938-1889786 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT192850 NZ_CP070048:4432810-4432886 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin