192846

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3028162..3028961 Replicon chromosome
Accession NZ_CP070048
Organism Escherichia coli strain FDAARGOS_1290

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag I6K20_RS14735 Protein ID WP_000347269.1
Coordinates 3028497..3028961 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag I6K20_RS14730 Protein ID WP_001309780.1
Coordinates 3028162..3028497 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K20_RS14715 (3023947) 3023947..3024717 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6K20_RS14720 (3024733) 3024733..3026067 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K20_RS14725 (3026442) 3026442..3028013 + 1572 WP_001273738.1 galactarate dehydratase -
I6K20_RS14730 (3028162) 3028162..3028497 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K20_RS14735 (3028497) 3028497..3028961 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K20_RS14740 (3029016) 3029016..3029825 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6K20_RS14745 (3030074) 3030074..3031354 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K20_RS14750 (3031377) 3031377..3031850 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K20_RS14755 (3031861) 3031861..3032640 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K20_RS14760 (3032630) 3032630..3033508 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K20_RS14765 (3033526) 3033526..3033960 + 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3018920..3028961 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T192846 WP_000347269.1 NZ_CP070048:3028497-3028961 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T192846 NZ_CP092561:c2123284-2123075 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT192846 WP_001309780.1 NZ_CP070048:3028162-3028497 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192846 NZ_CP092561:c2123589-2123296 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References