Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2985186..2985913 Replicon chromosome
Accession NZ_CP070048
Organism Escherichia coli strain FDAARGOS_1290

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag I6K20_RS14515 Protein ID WP_000550189.1
Coordinates 2985599..2985913 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag I6K20_RS14510 Protein ID WP_000560253.1
Coordinates 2985186..2985602 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K20_RS14500 (2980601) 2980601..2982952 + 2352 WP_000695439.1 alpha-glucosidase -
I6K20_RS14505 (2983123) 2983123..2985141 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
I6K20_RS14510 (2985186) 2985186..2985602 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
I6K20_RS14515 (2985599) 2985599..2985913 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
I6K20_RS14520 (2986198) 2986198..2987334 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
I6K20_RS14525 (2987419) 2987419..2987922 + 504 WP_001309773.1 M48 family metallopeptidase -
I6K20_RS14530 (2987999) 2987999..2988682 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
I6K20_RS14535 (2988761) 2988761..2989747 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T192845 WP_000550189.1 NZ_CP070048:c2985913-2985599 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T192845 NZ_CP092561:c2102178-2101816 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT192845 WP_000560253.1 NZ_CP070048:c2985602-2985186 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT192845 NZ_CP092561:c2102345-2102175 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References