Detailed information of TA system TA192841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1710647..1711149
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: I6K20_RS08645
• Protein ID: WP_000767829.1
• Coordinates: 1710647..1710901 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: I6K20_RS08650
• Protein ID: WP_001259255.1
• Coordinates: 1710898..1711149 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS08615 (1705662)	1705662..1705889	-	228	WP_000985278.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
I6K20_RS08620 (1705903)	1705903..1706961	-	1059	WP_000492312.1	thiosulfate utilization transporter TsuA/YeeE	-
I6K20_RS08625 (1707140)	1707140..1708498	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
I6K20_RS08630 (1708488)	1708488..1708550	-	63	WP_010723108.1	membrane protein YoeI	-
I6K20_RS08635 (1708765)	1708765..1709694	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
I6K20_RS08640 (1709740)	1709740..1710564	-	825	WP_000754758.1	SDR family oxidoreductase	-
I6K20_RS08645 (1710647)	1710647..1710901	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
I6K20_RS08650 (1710898)	1710898..1711149	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
I6K20_RS08655 (1711432)	1711432..1711482	+	51	WP_001364200.1	his operon leader peptide	-
I6K20_RS08660 (1711628)	1711628..1712527	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
I6K20_RS08665 (1712533)	1712533..1713837	+	1305	WP_001355813.1	histidinol dehydrogenase	-
I6K20_RS08670 (1713834)	1713834..1714904	+	1071	WP_068862954.1	histidinol-phosphate transaminase	-
I6K20_RS08675 (1714904)	1714904..1715971	+	1068	WP_000080142.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T192841 WP_000767829.1 NZ_CP070048:c1710901-1710647 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T192841 NZ_CP092559:71532-71789 [Staphylococcus aureus]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT192841 WP_001259255.1 NZ_CP070048:c1711149-1710898 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT192841 NZ_CP092559:71270-71539 [Staphylococcus aureus]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0