Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1071511..1072073 Replicon chromosome
Accession NZ_CP070048
Organism Escherichia coli strain FDAARGOS_1290

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag I6K20_RS05435 Protein ID WP_000605676.1
Coordinates 1071795..1072073 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K20_RS05430 Protein ID WP_000781370.1
Coordinates 1071511..1071795 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K20_RS05415 (1066693) 1066693..1069740 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
I6K20_RS05420 (1069753) 1069753..1070637 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
I6K20_RS05425 (1070630) 1070630..1071283 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I6K20_RS05430 (1071511) 1071511..1071795 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K20_RS05435 (1071795) 1071795..1072073 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K20_RS05440 (1072259) 1072259..1073269 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
I6K20_RS05445 (1073403) 1073403..1075100 - 1698 WP_000433464.1 malate dehydrogenase -
I6K20_RS05450 (1075257) 1075257..1075394 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K20_RS05455 (1075496) 1075496..1075711 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K20_RS05460 (1076056) 1076056..1076487 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T192830 WP_000605676.1 NZ_CP070048:c1072073-1071795 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192830 NZ_CP092556:c2124805-2124596 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192830 WP_000781370.1 NZ_CP070048:c1071795-1071511 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192830 NZ_CP092556:c2125110-2124817 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References