Detailed information of TA system TA192820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3701554..3702151
• Replicon: chromosome
• Accession: NZ_CP070046
• Organism: Klebsiella pneumoniae strain FDAARGOS_1319

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: I6K49_RS17805
• Protein ID: WP_004893639.1
• Coordinates: 3701554..3701871 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: I6K49_RS17810
• Protein ID: WP_004142561.1
• Coordinates: 3701864..3702151 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K49_RS17790	3697254..3698681	-	1428	WP_004176980.1	MFS transporter	-
I6K49_RS17795	3698790..3699656	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
I6K49_RS17800	3700326..3701369	+	1044	WP_023279204.1	DUF2157 domain-containing protein	-
I6K49_RS17805	3701554..3701871	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K49_RS17810	3701864..3702151	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K49_RS17815	3702289..3702942	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
I6K49_RS17820	3703062..3703430	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
I6K49_RS17825	3703430..3704011	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
I6K49_RS17830	3704191..3705306	+	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
I6K49_RS17835	3705337..3705678	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
I6K49_RS17840	3705696..3705944	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T192820 WP_004893639.1 NZ_CP070046:3701554-3701871 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T192820 NZ_CP092554:1962879-1963340 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTTGAAGCGCACAATTACGGTGTACGTAATTTATATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT192820 WP_004142561.1 NZ_CP070046:3701864-3702151 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT192820 NZ_CP092554:1962543-1962866 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAGA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCATTTAGAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1