Detailed information of TA system TA192797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4422237..4422839
• Replicon: chromosome
• Accession: NZ_CP070045
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K33_RS22175
• Protein ID: WP_000897302.1
• Coordinates: 4422237..4422548 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K33_RS22180
• Protein ID: WP_000356397.1
• Coordinates: 4422549..4422839 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS22150 (4418151)	4418151..4418750	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6K33_RS22155 (4418744)	4418744..4419616	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K33_RS22160 (4419613)	4419613..4420050	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K33_RS22165 (4420095)	4420095..4421036	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K33_RS22170 (4421100)	4421100..4422008	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6K33_RS22175 (4422237)	4422237..4422548	+	312	WP_000897302.1	hypothetical protein	Toxin
I6K33_RS22180 (4422549)	4422549..4422839	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6K33_RS22185 (4423198)	4423198..4423476	+	279	WP_001296612.1	hypothetical protein	-
I6K33_RS22190 (4423872)	4423872..4424090	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K33_RS22195 (4424306)	4424306..4425235	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K33_RS22200 (4425232)	4425232..4425867	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K33_RS22205 (4425864)	4425864..4426766	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192797 WP_000897302.1 NZ_CP070045:4422237-4422548 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192797 NZ_CP092547:c2102980-2102618 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192797 WP_000356397.1 NZ_CP070045:4422549-4422839 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192797 NZ_CP092547:c2103147-2102977 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: S1P416