Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2250740..2251242 Replicon chromosome
Accession NZ_CP070045
Organism Escherichia coli strain FDAARGOS_1303

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K33_RS11675 Protein ID WP_000767822.1
Coordinates 2250740..2250994 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K33_RS11680 Protein ID WP_001259255.1
Coordinates 2250991..2251242 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K33_RS11655 (2247233) 2247233..2248591 - 1359 WP_204672534.1 APC family permease -
I6K33_RS11660 (2248581) 2248581..2248643 - 63 WP_010723108.1 membrane protein YoeI -
I6K33_RS11665 (2248858) 2248858..2249787 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K33_RS11670 (2249833) 2249833..2250657 - 825 WP_000754733.1 SDR family oxidoreductase -
I6K33_RS11675 (2250740) 2250740..2250994 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K33_RS11680 (2250991) 2250991..2251242 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K33_RS11685 (2251525) 2251525..2251575 + 51 WP_001364200.1 his operon leader peptide -
I6K33_RS11690 (2251721) 2251721..2252620 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K33_RS11695 (2252626) 2252626..2253930 + 1305 WP_001297912.1 histidinol dehydrogenase -
I6K33_RS11700 (2253927) 2253927..2254997 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6K33_RS11705 (2254997) 2254997..2256064 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T192794 WP_000767822.1 NZ_CP070045:c2250994-2250740 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T192794 NZ_CP092547:c852447-852259 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT192794 WP_001259255.1 NZ_CP070045:c2251242-2250991 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT192794 NZ_CP092547:c852736-852476 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References