Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1620307..1620869 Replicon chromosome
Accession NZ_CP070045
Organism Escherichia coli strain FDAARGOS_1303

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K33_RS08625 Protein ID WP_000605675.1
Coordinates 1620591..1620869 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K33_RS08620 Protein ID WP_000781370.1
Coordinates 1620307..1620591 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K33_RS08605 (1615488) 1615488..1618535 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
I6K33_RS08610 (1618548) 1618548..1619432 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K33_RS08615 (1619425) 1619425..1620078 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K33_RS08620 (1620307) 1620307..1620591 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K33_RS08625 (1620591) 1620591..1620869 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K33_RS08630 (1621055) 1621055..1622065 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K33_RS08635 (1622199) 1622199..1623896 - 1698 Protein_1604 malate dehydrogenase -
I6K33_RS08640 (1624052) 1624052..1624189 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K33_RS08645 (1624291) 1624291..1624506 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K33_RS08650 (1624851) 1624851..1625282 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192783 WP_000605675.1 NZ_CP070045:c1620869-1620591 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192783 NZ_CP092542:c2416187-2415921 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192783 WP_000781370.1 NZ_CP070045:c1620591-1620307 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192783 NZ_CP092542:c2416438-2416187 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References