Detailed information of TA system TA192775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 249002..249696
• Replicon: chromosome
• Accession: NZ_CP070045
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K33_RS01690
• Protein ID: WP_001263500.1
• Coordinates: 249298..249696 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K33_RS01685
• Protein ID: WP_000554758.1
• Coordinates: 249002..249295 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS01660 (244538)	244538..245035	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6K33_RS01665 (245118)	245118..245276	-	159	WP_014639450.1	hypothetical protein	-
I6K33_RS01670 (245355)	245355..247067	-	1713	Protein_222	flagellar biosynthesis protein FlhA	-
I6K33_RS01675 (247039)	247039..247824	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K33_RS01680 (247895)	247895..248950	+	1056	WP_001226168.1	DNA polymerase IV	-
I6K33_RS01685 (249002)	249002..249295	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K33_RS01690 (249298)	249298..249696	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K33_RS01695 (249706)	249706..250158	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K33_RS01700 (250348)	250348..251487	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K33_RS01705 (251484)	251484..252098	+	615	WP_000602123.1	peptide chain release factor H	-
I6K33_RS01710 (252155)	252155..253612	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K33_RS01715 (253873)	253873..254331	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T192775 WP_001263500.1 NZ_CP070045:249298-249696 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192775 NZ_CP092540:c2442872-2442606 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192775 WP_000554758.1 NZ_CP070045:249002-249295 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192775 NZ_CP092540:c2443123-2442872 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3