Detailed information of TA system TA192772

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66825..67251
• Replicon: plasmid unnamed
• Accession: NZ_CP070044
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K33_RS00390
• Protein ID: WP_001372321.1
• Coordinates: 66825..66950 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67027..67251 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS00365 (62221)	62221..62910	-	690	WP_033550311.1	conjugal transfer transcriptional regulator TraJ	-
I6K33_RS00370 (63097)	63097..63480	-	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K33_RS00375 (63894)	63894..64403	+	510	WP_000759173.1	transglycosylase SLT domain-containing protein	-
I6K33_RS00380 (64700)	64700..65521	-	822	WP_001234445.1	DUF932 domain-containing protein	-
I6K33_RS00385 (65631)	65631..65927	-	297	WP_001272251.1	hypothetical protein	-
I6K33_RS25950 (66227)	66227..66524	+	298	Protein_77	hypothetical protein	-
I6K33_RS00390 (66825)	66825..66950	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K33_RS25955 (66892)	66892..67041	-	150	Protein_79	plasmid maintenance protein Mok	-
I6K33_RS00395 (67063)	67063..67242	+	180	WP_001309233.1	hypothetical protein	-
- (67027)	67027..67251	-	225	NuclAT_0	-	Antitoxin
- (67027)	67027..67251	-	225	NuclAT_0	-	Antitoxin
- (67027)	67027..67251	-	225	NuclAT_0	-	Antitoxin
- (67027)	67027..67251	-	225	NuclAT_0	-	Antitoxin
I6K33_RS00400 (67220)	67220..67982	-	763	Protein_81	plasmid SOS inhibition protein A	-
I6K33_RS00405 (67979)	67979..68413	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6K33_RS00410 (68482)	68482..70446	-	1965	WP_033550309.1	ParB/RepB/Spo0J family partition protein	-
I6K33_RS00415 (70507)	70507..70740	-	234	WP_033550308.1	DUF905 family protein	-
I6K33_RS00420 (70798)	70798..71325	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
I6K33_RS25960 (71627)	71627..72076	+	450	Protein_86	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86762	86762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192772 WP_001372321.1 NZ_CP070044:c66950-66825 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192772 NZ_CP092540:1963198-1963659 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTTGAAGCGCACAATTACGGTGTACGTAATTTATATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT192772 NZ_CP070044:c67251-67027 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin