Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4400390..4401117 Replicon chromosome
Accession NZ_CP070041
Organism Escherichia coli strain FDAARGOS_1293

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag I6K23_RS21120 Protein ID WP_000547555.1
Coordinates 4400390..4400701 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag I6K23_RS21125 Protein ID WP_000126293.1
Coordinates 4400698..4401117 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K23_RS21090 (4395531) 4395531..4397240 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
I6K23_RS21095 (4397250) 4397250..4397792 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
I6K23_RS21100 (4397792) 4397792..4398559 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6K23_RS21105 (4398556) 4398556..4398966 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I6K23_RS21110 (4398959) 4398959..4399429 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
I6K23_RS21115 (4399472) 4399472..4400227 + 756 WP_001305268.1 hypothetical protein -
I6K23_RS21120 (4400390) 4400390..4400701 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
I6K23_RS21125 (4400698) 4400698..4401117 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
I6K23_RS21130 (4401209) 4401209..4401637 - 429 WP_000536064.1 hypothetical protein -
I6K23_RS21135 (4402022) 4402022..4402549 + 528 WP_001078777.1 electron transport protein HydN -
I6K23_RS21140 (4402702) 4402702..4404954 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T192766 WP_000547555.1 NZ_CP070041:4400390-4400701 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T192766 NZ_CP092538:c2123787-2123578 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT192766 WP_000126293.1 NZ_CP070041:4400698-4401117 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT192766 NZ_CP092538:c2124092-2123799 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References