Detailed information of TA system TA192763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3806602..3807401
• Replicon: chromosome
• Accession: NZ_CP070041
• Organism: Escherichia coli strain FDAARGOS_1293

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: I6K23_RS18345
• Protein ID: WP_000347252.1
• Coordinates: 3806602..3807066 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: I6K23_RS18350
• Protein ID: WP_001296435.1
• Coordinates: 3807066..3807401 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K23_RS18315 (3801603)	3801603..3802037	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
I6K23_RS18320 (3802055)	3802055..3802933	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
I6K23_RS18325 (3802923)	3802923..3803702	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
I6K23_RS18330 (3803713)	3803713..3804186	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6K23_RS18335 (3804209)	3804209..3805489	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6K23_RS18340 (3805738)	3805738..3806547	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6K23_RS18345 (3806602)	3806602..3807066	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6K23_RS18350 (3807066)	3807066..3807401	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6K23_RS18355 (3807550)	3807550..3809121	-	1572	WP_001273940.1	galactarate dehydratase	-
I6K23_RS18360 (3809496)	3809496..3810830	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
I6K23_RS18365 (3810846)	3810846..3811616	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T192763 WP_000347252.1 NZ_CP070041:c3807066-3806602 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T192763 NZ_CP092538:c1889556-1889404 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT192763 WP_001296435.1 NZ_CP070041:c3807401-3807066 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT192763 NZ_CP092538:c1890180-1889581 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5