Detailed information of TA system TA192762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2968802..2969404
• Replicon: chromosome
• Accession: NZ_CP070041
• Organism: Escherichia coli strain FDAARGOS_1293

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K23_RS14300
• Protein ID: WP_000897302.1
• Coordinates: 2969093..2969404 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K23_RS14295
• Protein ID: WP_000356397.1
• Coordinates: 2968802..2969092 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K23_RS14270 (2964875)	2964875..2965777	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K23_RS14275 (2965774)	2965774..2966409	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K23_RS14280 (2966406)	2966406..2967335	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K23_RS14285 (2967551)	2967551..2967769	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K23_RS14290 (2968165)	2968165..2968443	-	279	WP_001296612.1	hypothetical protein	-
I6K23_RS14295 (2968802)	2968802..2969092	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K23_RS14300 (2969093)	2969093..2969404	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K23_RS14305 (2969634)	2969634..2970542	+	909	WP_001305059.1	alpha/beta hydrolase	-
I6K23_RS14310 (2970606)	2970606..2971547	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K23_RS14315 (2971592)	2971592..2972029	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K23_RS14320 (2972026)	2972026..2972898	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K23_RS14325 (2972892)	2972892..2973491	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192762 WP_000897302.1 NZ_CP070041:c2969404-2969093 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192762 NZ_CP092538:c852087-851899 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192762 WP_000356397.1 NZ_CP070041:c2969092-2968802 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192762 NZ_CP092538:c852376-852116 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: S1P416