Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 751467..752029 Replicon chromosome
Accession NZ_CP070041
Organism Escherichia coli strain FDAARGOS_1293

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K23_RS03590 Protein ID WP_000605675.1
Coordinates 751467..751745 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K23_RS03595 Protein ID WP_000781370.1
Coordinates 751745..752029 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K23_RS03565 (747053) 747053..747484 - 432 WP_000152307.1 peroxiredoxin OsmC -
I6K23_RS03570 (747829) 747829..748044 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K23_RS03575 (748146) 748146..748283 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K23_RS03580 (748440) 748440..750137 + 1698 WP_000433460.1 malate dehydrogenase -
I6K23_RS03585 (750271) 750271..751281 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K23_RS03590 (751467) 751467..751745 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K23_RS03595 (751745) 751745..752029 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K23_RS03600 (752258) 752258..752911 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I6K23_RS03605 (752904) 752904..753788 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K23_RS03610 (753801) 753801..756848 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192752 WP_000605675.1 NZ_CP070041:751467-751745 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192752 NZ_CP092536:801081-801491 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192752 WP_000781370.1 NZ_CP070041:751745-752029 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192752 NZ_CP092536:800834-801100 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References