Detailed information of TA system TA192702

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3220760..3221131
• Replicon: chromosome
• Accession: NZ_CP070026
• Organism: Escherichia coli strain FDAARGOS_1337

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: I6K67_RS15675
• Protein ID: WP_001317028.1
• Coordinates: 3220937..3221131 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3220760..3220938 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K67_RS24540 (3216512)	3216512..3216685	+	174	WP_001296046.1	protein YnaL	-
I6K67_RS15650 (3216715)	3216715..3218088	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
I6K67_RS15655 (3218217)	3218217..3219152	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I6K67_RS15660 (3219204)	3219204..3220439	-	1236	Protein_3019	site-specific integrase	-
I6K67_RS15665 (3220441)	3220441..3220656	-	216	WP_000079604.1	excisionase XisR	-
- (3220760)	3220760..3220938	+	179	NuclAT_0	-	Antitoxin
- (3220760)	3220760..3220938	+	179	NuclAT_0	-	Antitoxin
- (3220760)	3220760..3220938	+	179	NuclAT_0	-	Antitoxin
- (3220760)	3220760..3220938	+	179	NuclAT_0	-	Antitoxin
I6K67_RS15670 (3220735)	3220735..3220944	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
I6K67_RS15675 (3220937)	3220937..3221131	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I6K67_RS15680 (3221188)	3221188..3221997	-	810	WP_000166319.1	recombination protein RecT	-
I6K67_RS15685 (3221990)	3221990..3224590	-	2601	WP_044502199.1	exodeoxyribonuclease VIII	-
I6K67_RS15690 (3224692)	3224692..3224967	-	276	WP_000632297.1	protein RacC	-
I6K67_RS15695 (3225042)	3225042..3225212	-	171	WP_001352098.1	YdaE family protein	-
I6K67_RS15700 (3225212)	3225212..3225433	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3219204..3236180	16976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T192702 WP_001317028.1 NZ_CP070026:c3221131-3220937 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T192702 NZ_CP092532:c3492526-3492224 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT192702 NZ_CP070026:3220760-3220938 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin