Detailed information of TA system TA192700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2085158..2085852
• Replicon: chromosome
• Accession: NZ_CP070026
• Organism: Escherichia coli strain FDAARGOS_1337

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: I6K67_RS10155
• Protein ID: WP_001263489.1
• Coordinates: 2085454..2085852 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K67_RS10150
• Protein ID: WP_000554758.1
• Coordinates: 2085158..2085451 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K67_RS10125 (2080975)	2080975..2081442	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
I6K67_RS10130 (2081462)	2081462..2082178	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
I6K67_RS10135 (2082191)	2082191..2083054	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
I6K67_RS10140 (2083057)	2083057..2083980	+	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
I6K67_RS10145 (2084051)	2084051..2085106	+	1056	WP_001226164.1	DNA polymerase IV	-
I6K67_RS10150 (2085158)	2085158..2085451	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K67_RS10155 (2085454)	2085454..2085852	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K67_RS10160 (2085862)	2085862..2086314	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
I6K67_RS10165 (2086632)	2086632..2086838	+	207	Protein_1930	RtcB family protein	-
I6K67_RS10170 (2086834)	2086834..2087355	+	522	Protein_1931	peptide chain release factor H	-
I6K67_RS10175 (2087412)	2087412..2088869	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
I6K67_RS10180 (2089130)	2089130..2089588	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (2090184)	2090184..2090264	+	81	NuclAT_12	-	-
- (2090184)	2090184..2090264	+	81	NuclAT_12	-	-
- (2090184)	2090184..2090264	+	81	NuclAT_12	-	-
- (2090184)	2090184..2090264	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T192700 WP_001263489.1 NZ_CP070026:2085454-2085852 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192700 NZ_CP092532:2450598-2450876 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192700 WP_000554758.1 NZ_CP070026:2085158-2085451 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192700 NZ_CP092532:2450876-2451160 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3