Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2043607..2044148 Replicon chromosome
Accession NZ_CP070026
Organism Escherichia coli strain FDAARGOS_1337

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag I6K67_RS09915 Protein ID WP_000615983.1
Coordinates 2043607..2043885 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag I6K67_RS09920 Protein ID WP_000729703.1
Coordinates 2043888..2044148 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K67_RS09900 (2041189) 2041189..2041767 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
I6K67_RS09905 (2041973) 2041973..2042740 + 768 WP_000333380.1 class II glutamine amidotransferase -
I6K67_RS09910 (2042711) 2042711..2043451 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
I6K67_RS09915 (2043607) 2043607..2043885 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
I6K67_RS09920 (2043888) 2043888..2044148 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
I6K67_RS09925 (2044400) 2044400..2045107 + 708 WP_251301848.1 C40 family peptidase -
I6K67_RS09930 (2045283) 2045283..2045780 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
I6K67_RS09935 (2046004) 2046004..2048097 - 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / rhs/PAAR / gmhA/lpcA 2023658..2045780 22122


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T192699 WP_000615983.1 NZ_CP070026:c2043885-2043607 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T192699 NZ_CP092532:2130912-2131014 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT192699 WP_000729703.1 NZ_CP070026:c2044148-2043888 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT192699 NZ_CP092532:c2131052-2130784 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References