Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1376..2022 Replicon plasmid unnamed3
Accession NZ_CP070025
Organism Escherichia coli strain FDAARGOS_1337

Toxin (Protein)


Gene name higB Uniprot ID A0A3P1YQM6
Locus tag I6K67_RS00015 Protein ID WP_025667955.1
Coordinates 1376..1723 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag I6K67_RS00020 Protein ID WP_001259436.1
Coordinates 1723..2022 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K67_RS00005 (I6K67_00005) 557..844 + 288 WP_000356589.1 hypothetical protein -
I6K67_RS00010 (I6K67_00010) 868..1131 + 264 WP_000424604.1 hypothetical protein -
I6K67_RS00015 (I6K67_00015) 1376..1723 + 348 WP_025667955.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K67_RS00020 (I6K67_00020) 1723..2022 + 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
I6K67_RS00025 (I6K67_00025) 2186..2566 + 381 WP_000061763.1 hypothetical protein -
I6K67_RS00030 (I6K67_00030) 2630..2902 + 273 WP_204691431.1 helix-turn-helix transcriptional regulator -
I6K67_RS00035 (I6K67_00035) 3513..3701 + 189 WP_025667959.1 hypothetical protein -
I6K67_RS00040 (I6K67_00040) 3712..4887 - 1176 WP_025667960.1 ORF6N domain-containing protein -
I6K67_RS24155 4884..5171 - 288 WP_001673384.1 ash family protein -
I6K67_RS00050 (I6K67_00050) 5527..6099 + 573 WP_042968909.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48850 48850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13530.50 Da        Isoelectric Point: 9.3634

>T192692 WP_025667955.1 NZ_CP070025:1376-1723 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

>T192692 NZ_CP092532:c618802-618338 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT192692 WP_001259436.1 NZ_CP070025:1723-2022 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp

>AT192692 NZ_CP092532:c619137-618802 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P1YQM6

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References