Detailed information of TA system TA192654

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31160..31424
• Replicon: plasmid unnamed2
• Accession: NZ_CP070009
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: I6K18_RS26970
• Protein ID: WP_001303307.1
• Coordinates: 31160..31312 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31362..31424 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS26945 (26468)	26468..28636	+	2169	WP_000698357.1	DotA/TraY family protein	-
I6K18_RS26950 (28710)	28710..29360	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
I6K18_RS26955 (29432)	29432..29641	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
I6K18_RS26960 (30033)	30033..30209	+	177	WP_001054897.1	hypothetical protein	-
I6K18_RS28600 (30274)	30274..30369	-	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
I6K18_RS26965 (30879)	30879..31088	+	210	WP_000534733.1	hypothetical protein	-
I6K18_RS26970 (31160)	31160..31312	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
- (31362)	31362..31424	+	63	NuclAT_0	-	Antitoxin
- (31362)	31362..31424	+	63	NuclAT_0	-	Antitoxin
- (31362)	31362..31424	+	63	NuclAT_0	-	Antitoxin
- (31362)	31362..31424	+	63	NuclAT_0	-	Antitoxin
I6K18_RS26975 (31604)	31604..32812	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
I6K18_RS26980 (32831)	32831..33901	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
I6K18_RS26985 (33894)	33894..36185	+	2292	WP_001289276.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86286	86286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T192654 WP_001303307.1 NZ_CP070009:c31312-31160 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T192654 NZ_CP092500:991074-991385 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT192654 NZ_CP070009:31362-31424 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin