Detailed information of TA system TA192648

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 92622..93048
• Replicon: plasmid unnamed1
• Accession: NZ_CP070008
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K18_RS26125
• Protein ID: WP_001372321.1
• Coordinates: 92622..92747 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	92824..93048 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS26085 (87733)	87733..87960	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
I6K18_RS28415 (88048)	88048..88726	-	679	Protein_92	PAS domain-containing protein	-
I6K18_RS26100 (88860)	88860..89243	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K18_RS26105 (89586)	89586..90176	+	591	WP_001376243.1	transglycosylase SLT domain-containing protein	-
I6K18_RS26110 (90473)	90473..91294	-	822	WP_001234469.1	DUF932 domain-containing protein	-
I6K18_RS26115 (91413)	91413..91700	-	288	WP_000107535.1	hypothetical protein	-
I6K18_RS26120 (91725)	91725..91931	-	207	WP_000275859.1	hypothetical protein	-
I6K18_RS28420 (92001)	92001..92174	+	174	Protein_98	hypothetical protein	-
I6K18_RS28425 (92172)	92172..92402	-	231	WP_001426396.1	hypothetical protein	-
I6K18_RS26125 (92622)	92622..92747	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K18_RS28430 (92689)	92689..92838	-	150	Protein_101	plasmid maintenance protein Mok	-
- (92824)	92824..93048	-	225	NuclAT_0	-	Antitoxin
- (92824)	92824..93048	-	225	NuclAT_0	-	Antitoxin
- (92824)	92824..93048	-	225	NuclAT_0	-	Antitoxin
- (92824)	92824..93048	-	225	NuclAT_0	-	Antitoxin
I6K18_RS26130 (92860)	92860..93048	+	189	WP_001299721.1	hypothetical protein	-
I6K18_RS26135 (93017)	93017..93779	-	763	Protein_103	plasmid SOS inhibition protein A	-
I6K18_RS26140 (93776)	93776..94210	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
I6K18_RS26145 (94265)	94265..96223	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
I6K18_RS26150 (96289)	96289..96522	-	234	WP_000005990.1	DUF905 family protein	-
I6K18_RS26155 (96585)	96585..97124	-	540	WP_000290841.1	single-stranded DNA-binding protein	-
I6K18_RS28435 (97150)	97150..97356	-	207	WP_000547975.1	hypothetical protein	-
I6K18_RS28440 (97597)	97597..97878	-	282	WP_072277694.1	hypothetical protein	-
I6K18_RS26160 (97767)	97767..98003	-	237	WP_032153960.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..198318	198318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192648 WP_001372321.1 NZ_CP070008:c92747-92622 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192648 NZ_CP092499:477213-477563 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT192648 NZ_CP070008:c93048-92824 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin