Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4475167..4475729 Replicon chromosome
Accession NZ_CP070007
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag I6K18_RS21570 Protein ID WP_000605676.1
Coordinates 4475167..4475445 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K18_RS21575 Protein ID WP_000781370.1
Coordinates 4475445..4475729 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS21545 (4470753) 4470753..4471184 - 432 WP_000152305.1 peroxiredoxin OsmC -
I6K18_RS21550 (4471529) 4471529..4471744 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K18_RS21555 (4471846) 4471846..4471983 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K18_RS21560 (4472140) 4472140..4473837 + 1698 WP_000433464.1 malate dehydrogenase -
I6K18_RS21565 (4473971) 4473971..4474981 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
I6K18_RS21570 (4475167) 4475167..4475445 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K18_RS21575 (4475445) 4475445..4475729 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K18_RS21580 (4475957) 4475957..4476610 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I6K18_RS21585 (4476603) 4476603..4477487 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
I6K18_RS21590 (4477500) 4477500..4480547 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T192640 WP_000605676.1 NZ_CP070007:4475167-4475445 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192640 NZ_CP092493:c4647318-4647016 [Citrobacter freundii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACTAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192640 WP_000781370.1 NZ_CP070007:4475445-4475729 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192640 NZ_CP092493:c4646989-4646648 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References