Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2478333..2479060 Replicon chromosome
Accession NZ_CP070007
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag I6K18_RS11930 Protein ID WP_000550189.1
Coordinates 2478333..2478647 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag I6K18_RS11935 Protein ID WP_000560253.1
Coordinates 2478644..2479060 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS11910 (2474499) 2474499..2475485 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
I6K18_RS11915 (2475564) 2475564..2476247 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
I6K18_RS11920 (2476324) 2476324..2476827 - 504 WP_001309773.1 M48 family metallopeptidase -
I6K18_RS11925 (2476912) 2476912..2478048 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
I6K18_RS11930 (2478333) 2478333..2478647 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
I6K18_RS11935 (2478644) 2478644..2479060 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
I6K18_RS11940 (2479105) 2479105..2481123 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
I6K18_RS11945 (2481294) 2481294..2483645 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T192627 WP_000550189.1 NZ_CP070007:2478333-2478647 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T192627 NZ_CP092493:c76534-76193 [Citrobacter freundii]
ATGAAACCTCAACCTGCAACAACTTCGCGGGCGGCTGAGCTATATCTGTCGCCAGTGGCGGTGTGGCAAATGCTACTGAC
ACGCCTGCTGGAGCAGCACTATGGCCTGACACTGAACGACACGCCATTTAGTGATGAAACCGTTATTCAGGAACACATTG
ATGCCGGGATCATGCTGGCCGATGCTGTGAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCGCCTTATCTTCGGGCTGTAGATATTTTACGGGCAAGACAGGCAACTGGCCTGTTGCGACAAAG
CCGTAACAACGCAGTACGGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT192627 WP_000560253.1 NZ_CP070007:2478644-2479060 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT192627 NZ_CP092493:c76875-76555 [Citrobacter freundii]
ATGAGAAGTAACATCACATGGGGCCTGCAGCGGGATATCACGCCGTGCATGGGAGCACGTCTGGTGCAGGAGGGTAACCG
GCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGACGAATGTCTTAAGCTGGATGTGGTATTTC
CACATTTTATCAGCCGGATCGAGTCTATGCTGACCACTGGTGAATTGAATCCCCGTCATGCCCACTGCATCACCATTTAC
CACAACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGCCTACGTATATGTAGTCGTCTATTCCACTCAACGCTA
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References