Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2434509..2435308 Replicon chromosome
Accession NZ_CP070007
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag I6K18_RS11705 Protein ID WP_000347269.1
Coordinates 2434509..2434973 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag I6K18_RS11710 Protein ID WP_001309780.1
Coordinates 2434973..2435308 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS11675 (2429510) 2429510..2429944 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
I6K18_RS11680 (2429962) 2429962..2430840 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K18_RS11685 (2430830) 2430830..2431609 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K18_RS11690 (2431620) 2431620..2432093 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K18_RS11695 (2432116) 2432116..2433396 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K18_RS11700 (2433645) 2433645..2434454 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6K18_RS11705 (2434509) 2434509..2434973 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K18_RS11710 (2434973) 2434973..2435308 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K18_RS11715 (2435457) 2435457..2437028 - 1572 WP_001273738.1 galactarate dehydratase -
I6K18_RS11720 (2437403) 2437403..2438737 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K18_RS11725 (2438753) 2438753..2439523 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T192626 WP_000347269.1 NZ_CP070007:c2434973-2434509 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T192626 NZ_CP092493:44529-44891 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGTAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT192626 WP_001309780.1 NZ_CP070007:c2435308-2434973 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192626 NZ_CP092493:44878-45177 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References