Detailed information of TA system TA192616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 624936..625586
• Replicon: chromosome
• Accession: NZ_CP070007
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6K18_RS03120
• Protein ID: WP_000263532.1
• Coordinates: 625245..625586 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: I6K18_RS03115
• Protein ID: WP_000212552.1
• Coordinates: 624936..625235 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS03090 (620384)	620384..620695	-	312	WP_000415004.1	hypothetical protein	-
I6K18_RS03095 (620700)	620700..621092	-	393	WP_000285322.1	flagellar export chaperone FliS	-
I6K18_RS03100 (621115)	621115..622431	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
I6K18_RS03105 (622636)	622636..623550	-	915	WP_000949083.1	lateral flagellin LafA	-
I6K18_RS03110 (624036)	624036..624878	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
I6K18_RS03115 (624936)	624936..625235	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K18_RS03120 (625245)	625245..625586	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K18_RS03125 (625662)	625662..626639	-	978	WP_001195938.1	hypothetical protein	-
I6K18_RS03130 (626656)	626656..627585	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
I6K18_RS03135 (627600)	627600..628976	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
I6K18_RS03140 (629152)	629152..629451	-	300	WP_000867281.1	rod-binding protein	-
I6K18_RS03145 (629451)	629451..630551	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T192616 WP_000263532.1 NZ_CP070007:c625586-625245 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T192616 NZ_CP092478:886387-886752 [Staphylococcus haemolyticus]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTGTCACCAGTACAGGGCTCAGAACAAGGGGGAGTCAGACCTGTAGT
TATCATTCAAAACGATACTGGTAACAAATATAGTCCTACCGTAATCGTTGCTGCGATTACGGGAAGGATTAATAAAGCGA
AAATACCCACACATGTTGAAATCGAGAAAAAGAAATACAGACTTGATAAAGATTCAGTTATTCTACTCGAGCAAATTAGA
ACATTAGATAAAAAACGACTAAAAGAAAAATTAACATATCTATCTGATGAGAAAATGCAAGAAGTGGATGAAGCGTTAGA
CATTAGTCTAGGATTGCATGATGAAGTTAAAACGCAAAACACATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT192616 WP_000212552.1 NZ_CP070007:c625235-624936 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT192616 NZ_CP092478:886220-886390 [Staphylococcus haemolyticus]
ATGCTTACTTTCAATCAAAATAGAAGTCACAGTCTTGAACAATCTTTAAAAGAAGGCTATGCACAAATGGCCGAATTAAA
TCTCTCCCTAGCGACAGAAGCTTTTCCTCTAGAATGCGAAGCTTGTGATTGCAACGAAACGTACCTTATTTCTAATTATA
AAAGTGAATGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7