Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4823275..4823837 Replicon chromosome
Accession NZ_CP069996
Organism Escherichia coli strain FDAARGOS_1300

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K30_RS23520 Protein ID WP_000605675.1
Coordinates 4823275..4823553 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K30_RS23525 Protein ID WP_000781370.1
Coordinates 4823553..4823837 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K30_RS23495 (4818862) 4818862..4819293 - 432 WP_000152310.1 peroxiredoxin OsmC -
I6K30_RS23500 (4819638) 4819638..4819853 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K30_RS23505 (4819955) 4819955..4820092 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K30_RS23510 (4820248) 4820248..4821945 + 1698 WP_000433462.1 malate dehydrogenase -
I6K30_RS23515 (4822079) 4822079..4823089 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K30_RS23520 (4823275) 4823275..4823553 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K30_RS23525 (4823553) 4823553..4823837 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K30_RS23530 (4824066) 4824066..4824719 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K30_RS23535 (4824712) 4824712..4825596 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K30_RS23540 (4825609) 4825609..4828656 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192611 WP_000605675.1 NZ_CP069996:4823275-4823553 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192611 NZ_CP092475:894961-895113 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192611 WP_000781370.1 NZ_CP069996:4823553-4823837 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192611 NZ_CP092475:894337-894936 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References