Detailed information of TA system TA192600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4252553..4253055
• Replicon: chromosome
• Accession: NZ_CP069996
• Organism: Escherichia coli strain FDAARGOS_1300

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: I6K30_RS20570
• Protein ID: WP_000767829.1
• Coordinates: 4252801..4253055 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: I6K30_RS20565
• Protein ID: WP_001259255.1
• Coordinates: 4252553..4252804 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K30_RS20540 (4247731)	4247731..4248798	-	1068	WP_001513846.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
I6K30_RS20545 (4248798)	4248798..4249868	-	1071	WP_016239284.1	histidinol-phosphate transaminase	-
I6K30_RS20550 (4249865)	4249865..4251169	-	1305	WP_001513845.1	histidinol dehydrogenase	-
I6K30_RS20555 (4251175)	4251175..4252074	-	900	WP_000131789.1	ATP phosphoribosyltransferase	-
I6K30_RS20560 (4252220)	4252220..4252270	-	51	WP_071593552.1	his operon leader peptide	-
I6K30_RS20565 (4252553)	4252553..4252804	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
I6K30_RS20570 (4252801)	4252801..4253055	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
I6K30_RS20575 (4253138)	4253138..4253962	+	825	WP_000754737.1	SDR family oxidoreductase	-
I6K30_RS20580 (4254008)	4254008..4254937	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
I6K30_RS20585 (4255152)	4255152..4255214	+	63	WP_010723108.1	membrane protein YoeI	-
I6K30_RS20590 (4255204)	4255204..4256562	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
I6K30_RS20595 (4256741)	4256741..4257799	+	1059	WP_000492340.1	thiosulfate utilization transporter TsuA/YeeE	-
I6K30_RS20600 (4257813)	4257813..4258040	+	228	WP_083574903.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T192600 WP_000767829.1 NZ_CP069996:4252801-4253055 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T192600 NZ_CP092467:c33967-33551 [Citrobacter portucalensis]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT192600 WP_001259255.1 NZ_CP069996:4252553-4252804 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT192600 NZ_CP092467:c34194-33964 [Citrobacter portucalensis]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0