Detailed information of TA system TA192579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 934258..934952
• Replicon: chromosome
• Accession: NZ_CP069996
• Organism: Escherichia coli strain FDAARGOS_1300

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K30_RS04590
• Protein ID: WP_001263500.1
• Coordinates: 934258..934656 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K30_RS04595
• Protein ID: WP_000554758.1
• Coordinates: 934659..934952 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K30_RS04565 (929623)	929623..930081	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
I6K30_RS04570 (930342)	930342..931799	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K30_RS04575 (931856)	931856..932470	-	615	WP_000602123.1	peptide chain release factor H	-
I6K30_RS04580 (932467)	932467..933606	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K30_RS04585 (933796)	933796..934248	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K30_RS04590 (934258)	934258..934656	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K30_RS04595 (934659)	934659..934952	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K30_RS04600 (935004)	935004..936059	-	1056	WP_001226168.1	DNA polymerase IV	-
I6K30_RS04605 (936130)	936130..936915	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K30_RS04610 (936887)	936887..938599	+	1713	Protein_907	flagellar biosynthesis protein FlhA	-
I6K30_RS04615 (938678)	938678..938836	+	159	WP_014639450.1	hypothetical protein	-
I6K30_RS04620 (938919)	938919..939416	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / vgrG/tssI	934258..954611	20353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T192579 WP_001263500.1 NZ_CP069996:c934656-934258 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192579 NZ_CP092461:55327-55602 [Serratia marcescens]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192579 WP_000554758.1 NZ_CP069996:c934952-934659 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192579 NZ_CP092461:55067-55327 [Serratia marcescens]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAGCGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3