Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 675252..675931 Replicon chromosome
Accession NZ_CP069996
Organism Escherichia coli strain FDAARGOS_1300

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6K30_RS03330 Protein ID WP_000057524.1
Coordinates 675629..675931 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K30_RS03325 Protein ID WP_000806442.1
Coordinates 675252..675593 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K30_RS03315 (671496) 671496..672428 - 933 WP_000883052.1 glutaminase A -
I6K30_RS03320 (672690) 672690..675194 + 2505 WP_016239198.1 copper-exporting P-type ATPase CopA -
I6K30_RS03325 (675252) 675252..675593 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K30_RS03330 (675629) 675629..675931 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K30_RS03335 (676064) 676064..676858 + 795 WP_000365177.1 TraB/GumN family protein -
I6K30_RS03340 (677062) 677062..677541 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K30_RS03345 (677565) 677565..678365 + 801 WP_000439796.1 hypothetical protein -
I6K30_RS03350 (678362) 678362..678865 + 504 WP_000667000.1 hypothetical protein -
I6K30_RS03355 (678903) 678903..680555 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 667941..678865 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T192577 WP_000057524.1 NZ_CP069996:c675931-675629 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T192577 NZ_CP092460:c2468468-2468388 [Dickeya fangzhongdai]
AATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGGGCACAGGAATCGTGTTCGGTCTTTTTT
T

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT192577 WP_000806442.1 NZ_CP069996:c675593-675252 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT192577 NZ_CP092460:2468387-2468468 [Dickeya fangzhongdai]
TAAAAAAAGACCGAACACGATTCCTGTGCCCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References